Analysis Note

Positive ControlRat brain

Application

ELISA (1:2500-1:10,000) r>Frozen Sections (see application references)r>Immunoblotting (1:200) r>Immunocytochemistry (1:500-1:2500) r>Immunoprecipitation (1 µg/200 µg lysate)

General description

This Anti-Synapsin I Rabbit pAb is validated for use in ELISA, Immunoblotting Immunocytochemistry Immunoprecipitation for the detection of Synapsin I.

Recognizes the synapsin I protein in rat brain.

Immunoaffinity purified rabbit polyclonal antibody. Recognizes the synapsin I protein.

Immunogen

purified, brovine brain synapsin I

Bovine

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Stone, L.M., et al. 1994. J. Neurosci.14, 301.Mandell, J.W., et al. 1992. J. Neurosci.12, 1736.Fletcher, T.L., et al. 1991. J. Neurosci.11, 1617.Moore, R.Y., and Berstein, M. 1989. J. Neurosci.9, 2151.

This antibody is specific for synapsin I and can be used to estimate synaptic density and synaptogenesis. Strong and specific immunolabeling of the synapsin I doublet seen in immunoblotting at a 1:200 dilution. This immunolabeling can be blocked by preadsorption of antibody with synapsin I. Variables associated with assay conditions will dictate the proper working dilution.

Packaging

10 µg in Plastic ampoule

Physical form

Lyophilized from 5 mM ammonium bicarbonate.

Reconstitution

Reconstitute with 50 µl PBS. Following reconstitution, aliquot and freeze (-20°C). Avoid freeze/thaw cycles of solutions.

Warning

Toxicity: Standard Handling (A)